Cryptic speciation in Triatoma sordida (Hemiptera: Reduviidae) from the Bolivian Chaco

Noireau F, Gutiérrez T, Zegarra M, Flores R, Breniere F, Dujardin JP

    Eight natural Bolivian populations of two closely related species of Triatominae, Triatoma sordida and T. guasayana, were analysed by multilocus enzyme electrophoresis at 20 loci. Both species were readily separated and no natural hybrid was observed. Among the silvatic sample of T. sordida, strong departure from panmixia within and between loci was detected in two sites of the Chaco, suggesting two reproductively separate populations easily recognized at ldh2 and Mdh2 loci. Genetic distance between them was in agreement with the hypothesis of distinct species. However, the detection of 3% of putative hybrids suggested a recent evolutionary divergence.

Key words:    Triatoma sordida, T. guasayana, multilocus enzyme electrophoresis (MLEE), Hardy-Weinberg disequilibrium, linkage disequilibrium, speciation.